BioDIP Winter Seminar on Fluorescence Lifetime Imaging Microscopy (FLIM)
FLIM example image from "Moderation of Arabidopsis root stemness by CLAVATA1 and ARABIDOPSIS CRINKLY4 receptor kinase complexes. Stahl Y, Grabowski S, Bleckmann A, Kühnemuth R, Weidtkamp-Peters S, Pinto KG, Kirschner GK, Schmid JB, Wink RH, Hülsewede A, Felekyan S, Seidel CA, Simon R. Curr Biol. 2013 Mar 4;23(5):362-71. doi: 10.1016/j.cub.2013.01.045."
The BioDIP Lighthouse Seminar Series was established to introduce a microscopy technique, available within the BioDIP, to local scientists and encourage interaction and exchange of information within the community. The seminar typically starts with a short introduction to the technique followed by 2 or 3 application talks, which put their focus mainly on the technical aspects.
This winter seminar is dedicated to Fluorescence Lifetime Imaging Microscopy (FLIM), a technique that e.g. allows to study protein-protein interactions in vivo.
The introduction will be given by Stefanie Weidkamp-Peters from the Center of Advanced Imaging of the Heinrich Heine University Düsseldorf. Stefanie has many years of experience in microscopy & spectroscopy and is used to clearly explain advanced technologies.
Moreover three colleagues who already applied FLIM in their research projects will give a practical insight into the pros & cons of the technology:
Julia Janssen (MTZ)
Sigrun Hoffmann (UKD)
Gokul Kesavan (BIOTEC/CRTD)
FLIM instrumentation is available at the LMF BIOTEC/CRTD.